Scanning Electron Microscopy Datasets -- Emiliania Huxleyi Strains From Naturally High And Low Co2 Waters Responding To High And Low Co2 In The Lab

Study question: How do Emiliania huxleyi strains isolated from naturally high CO2 waters or low CO2 waters respond to exposure to high and low CO2 levels? Associated article:<br> Peter von Dassow, Francisco Díaz-Rosas, El Mahdi Bendif, Juan-Diego Gaitán-Espitia, Daniella Mella-Flores, Sebastian Rokitta, Uwe John, and Rodrigo Torres. 2018. Over-calcified forms of the coccolithophore <em>Emiliania huxleyi </em>in high-CO2 waters are not preadapted to ocean acidification. Biogeosciences. https://doi.org/10.5194/bg-15-1-2018 Technical notes: Three electron microscopes were used: TM3000 (Hitachi High-Technologies, Tokyo, Japan) in the Unidad de Microscopía Avanzada of the Facultad de Ciencias Biológicas, Pontificia Univesidad Católica de Chile. The Hitachi microscope is not of high quality, and, when available, other electron microscopes were used. Quanta 250 (FEI, Hillsboro, Oregon, USA) in the Facultad de Geología, Universidad de Chile Quanta FEG 250 (FEI, Hillsboro, Oregon, USA) in the laboratory CIEN-UC, Facultad de Física, Pontificia Universidad Católica de Chile. Data set 1: Data-sharing-SEM_Calfuco-CO2 experiment.zip Scanning electron microscopy images of E. huxleyi strains after bubbling with 1200 µatm CO2 and 400 µatm CO2 air/CO2 mixes. Data set 2: Field-SEM-2011-2013.zip Scanning electron microscope images of filters of plankton samples taken during field campaigns. See article for methodology. For the samples from ElQuisco_2012 and JuanFernandez_2011, note that the last two digits in the sample name refer to the depth from which the sample was obtained (ej., “FQ.01.01.05D” is from 5 m and “FQ.01.01.15D” is from 15 m). Tables are provided to associate counts and taxonomic identifications to environmental variables from the samples for which data was used in statistical analysis. Note also that images do not correspond to all counts reported, as sometimes counts were made without capturing images due to time pressure for microscope use.研究问题：从天然高CO₂水体与低CO₂水体中分离的赫氏颗石藻（Emiliania huxleyi）菌株，在暴露于高、低CO₂水平时会产生何种响应？ 关联论文： Peter von Dassow、Francisco Díaz-Rosas、El Mahdi Bendif、Juan-Diego Gaitán-Espitia、Daniella Mella-Flores、Sebastian Rokitta、Uwe John与Rodrigo Torres，2018年。《高CO₂水体中过度钙化的颗石藻赫氏颗石藻（Emiliania huxleyi）并非预适应海洋酸化》。《生物地球科学》（Biogeosciences）。https://doi.org/10.5194/bg-15-1-2018 技术说明： 本研究共使用三台电子显微镜： 1. TM3000型扫描电子显微镜（日立高新技术公司，日本东京），部署于智利天主教大学生物科学学院高级显微学单元。该日立显微镜性能有限，如有条件时优先选用其他电子显微镜。 2. Quanta 250型扫描电子显微镜（FEI公司，美国俄勒冈州希尔斯伯勒），部署于智利大学地质学院。 3. Quanta FEG 250型扫描电子显微镜（FEI公司，美国俄勒冈州希尔斯伯勒），部署于智利天主教大学物理学院CIEN-UC实验室。 数据集1：Data-sharing-SEM_Calfuco-CO2实验.zip 该数据集包含赫氏颗石藻（Emiliania huxleyi）菌株在通入1200 µatm CO₂与400 µatm CO₂空气-CO₂混合气体后的扫描电子显微镜图像。 数据集2：Field-SEM-2011-2013.zip 该数据集包含野外采样获得的浮游生物样本滤膜的扫描电子显微镜图像。详细实验方法参见关联论文。针对ElQuisco_2012与JuanFernandez_2011的样本，需注意：样本名称的最后两位数字代表样本采集深度（例如，"FQ.01.01.05D"对应5米水深样本，"FQ.01.01.15D"对应15米水深样本）。本数据集附带表格，用于将统计分析中使用的样本的计数结果、分类鉴定信息与环境变量相关联。需注意：由于显微镜使用时长受限，部分样本仅完成计数未采集图像，因此图像并非覆盖所有报告的计数样本。