DataSheet4_Bu-Gu-Sheng-Sui decoction promotes osteogenesis via activating the ERK/Smad signaling pathways.zip

Osteoporosis is a systemic metabolic skeletal disease, which becomes a common public health problem that seriously endangers people’s health. Bu-Gu-Sheng-Sui decoction (BGSSD) is a safe and effective Chinese medicine formulation for the treatment of osteoporosis. Numerous studies have indicated that it played a significant role in bone anabolism. However, the underlying mechanism remains unclear. Herein, we selected senescence-accelerated mice prone 6 (SAMP6) and MC3T3-E1 cells to study the effects of BGSSD on osteogenesis and then investigated the potential mechanism of BGSSD. Our research found that BGSSD protected the bone mass in SAMP6, increased the expression of osteogenic specific factor Runx2, and improved bone trabecular structure. In vitro, BGSSD accelerated the proliferation and differentiation of MC3T3-E1 cells, which was characterized by stimulating the activity of Alkaline phosphatase (ALP) and raising the expression of Runx2. Moreover, BGSSD could effectively boost the expression levels of ERK and Smad in SAMP6 and MC3T3-E1. Therefore, we speculate that BGSSD may promote bone formation through ERK/Smad pathways. Collectively, our results highlight the importance of BGSSD as a compound in promoting osteogenic differentiation and osteogenesis, demonstrating that BGSSD may become a latent drug to prevent and treat osteoporosis.

骨质疏松症（Osteoporosis）是一种全身性代谢性骨骼疾病，现已成为严重危害人类健康的常见公共卫生问题。补骨生髓汤（Bu-Gu-Sheng-Sui decoction, BGSSD）是一种安全有效的骨质疏松症治疗用中药方剂，多项研究表明其在骨合成代谢中发挥着重要作用，但其具体作用机制仍未明确。本研究选取快速老化小鼠P6亚系（senescence-accelerated mice prone 6, SAMP6）与小鼠成骨细胞系MC3T3-E1，探究补骨生髓汤对骨生成的影响，并解析其潜在作用机制。研究结果显示，补骨生髓汤可维持快速老化小鼠P6亚系的骨量，上调成骨特异性因子Runx2的表达，并改善骨小梁结构。体外实验中，补骨生髓汤可促进MC3T3-E1细胞的增殖与分化，具体表现为增强碱性磷酸酶（Alkaline phosphatase, ALP）活性并上调Runx2的表达水平。此外，补骨生髓汤可有效上调快速老化小鼠P6亚系与MC3T3-E1细胞中ERK与Smad的表达水平。据此，我们推测补骨生髓汤可能通过ERK/Smad信号通路促进骨生成。综上，本研究结果证实了补骨生髓汤作为活性成分在促进成骨细胞分化与骨生成中的重要作用，表明其有望成为预防与治疗骨质疏松症的潜在药物。