Transcriptomic analysis of fungus Penicillium oxalicum and its set2 deletion strains

The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), and set2 knockout strain (?set2) in different carbon sources. The deletion of set2 upregulated genes involved in oxidation- reduction process, extracellular region, and plasma membrane ATP synthesis coupled proton transport both with 2% glucose or 1% cellulose and 1% wheat bran as carbon sources. We find the expression levels of 20 secondary metabolism gene clusters were upregulated or downregulated under different carbon sources in ?set2. This study provides the information that SET2 function are required in conidiation and hydrolase activity of P. oxalicum. Overall design: Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and set2 knockout mutant strains in 24h in modifeid Czapek culture medium with 2% glucose or 1% cellulose and 1% wheat bran as carbon sources.Three replicates were included for each samples.

本研究旨在比较草酸青霉（Penicillium oxalicum）野生型菌株（WT，wild type strain）与set2敲除菌株（Δset2）在不同碳源条件下的基因表达差异。在以2%葡萄糖、1%纤维素与1%麦麸为碳源的培养体系中，set2基因的缺失会上调参与氧化还原过程、细胞外区域以及质膜ATP合成耦联质子转运的相关基因。我们发现，在不同碳源条件下，Δset2菌株中共有20个次级代谢基因簇的表达水平出现上调或下调。本研究证实，SET2在草酸青霉的产孢过程与水解酶活性中发挥必需功能。整体实验设计：采用数字基因表达标签谱分析（digital gene expression tag profiling）技术，检测在以2%葡萄糖、1%纤维素与1%麦麸为碳源的改良察氏培养基（modified Czapek culture medium）中培养24小时的草酸青霉野生型菌株与set2敲除突变菌株的差异基因表达情况。每个样本设置3次生物学重复。