The cold exposure produce a different gene expression pattern in inguinal and periaortic white adipose

Male ferrets, aged 3 months, were divided into two group: one group remained at 22 degrees Celsius, while the other group was acclimatized to 4 degrees Celsius for one week. After sacrification, inguinal and periaortic white adipose tissues were dissected, and used for RNA isolation and subsequent global gene expression profiling using custom Agilent ferret-specific 2x400K microarrays. Data analysis indicated that while the cold exposure induces an increase on metabolism in inguinal white adopose tissue, in periaortic white adipose tissue this stimulus induces a reduction on expression of genes involved in cell cycle and in immune response. Ferrets, male, aged 3 months, were divided into two groups: one group remained at 22 degrees Celsius, while the other group was acclimatized to 4 degrees Celsius for one week. After sacrification, inguinal and periaortic white adipose tissues were dissected, and immediately snap frozen in liquid nitrogen. Total RNA was isolated, quantified and qualified, and subsequently used for global gene expression profiling using custom Agilent ferret-specific 2x400K microarrays.

将3月龄雄性雪貂分为两组：一组置于22摄氏度环境中常规饲养，另一组于4摄氏度环境中适应性驯化一周。处死后分离腹股沟白色脂肪组织（inguinal white adipose tissue）与主动脉旁白色脂肪组织（periaortic white adipose tissue），用于RNA提取及后续采用定制化安捷伦（Agilent）雪貂专属2×400K基因微阵列开展的全基因表达谱分析。
数据分析结果显示：寒冷暴露可使腹股沟白色脂肪组织的代谢相关基因表达上调，而在主动脉旁白色脂肪组织中，该冷刺激会下调参与细胞周期与免疫反应的基因的表达。
另有实验流程详述如下：将3月龄雄性雪貂分为两组：一组置于22摄氏度环境中常规饲养，另一组于4摄氏度环境中适应性驯化一周。处死后分离腹股沟白色脂肪组织与主动脉旁白色脂肪组织，立即置于液氮中快速冷冻。提取总RNA并对其浓度与质量进行定量及质控检测，随后将其用于全基因表达谱分析，所用检测平台为定制化安捷伦（Agilent）雪貂专属2×400K基因微阵列。