Table_1_Expression profile of long non-coding RNA in inner Mongolian cashmere goat with putative roles in hair follicles development.XLS

The hair follicle is a complex skin accessory organ, which determines hair growth. Long non-coding RNAs (lncRNAs) have been proven to play an important role in hair follicle development, but their specific mechanism is still unclear. In this study, high-throughput sequencing was used to obtain the expression profiles of lncRNA in the hair follicles of Inner Mongolian cashmere goats at different embryonic stages (45, 55, 65, and 75 days), and a total of 6,630 lncRNA were identified. According to the rules of hair follicle development, we combined miRNA and mRNA databases (published) and predicted lncRNA-miRNA, miRNA-mRNA, and lncRNA-mRNA interaction pairs in the 45 vs. 75 comparison group. We obtained 516 lncRNA-mRNA, 1,011 lncRNA-miRNA, and 7,411 miRNA-mRNA relationship pairs. Finally, target genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and it was found that they were mainly enriched in the Wnt signaling pathway and PI3K-Akt signaling pathway related to hair follicle development, indicating that lncRNA may interact with miRNA/mRNA to directly or indirectly regulate the expression of genes related to hair follicle development. Dual-luciferase reporter gene analysis showed that lncRNA MSTRG.1705.1 could bind to Chi-miR-1, while lncRNA MSTRG.11809.1 had no binding site for Chi-miR-433. In conclusion, this study aims to further analyze the molecular regulation mechanism of hair follicle development and to lay a theoretical foundation for revealing the regulation mechanism of cashmere hair follicle growth.

毛囊（hair follicle）是一类复杂的皮肤附属器官，其功能决定毛发的生长。长链非编码RNA（long non-coding RNAs, lncRNAs）已被证实可在毛囊发育过程中发挥重要调控作用，但具体分子机制尚未明确。本研究采用高通量测序技术，获取了不同胚胎发育阶段（45、55、65及75日龄）内蒙古绒山羊毛囊组织中lncRNA的表达谱，共鉴定得到6630个lncRNA。依据毛囊发育的时序规律，本研究结合已发表的微小RNA（miRNA）及信使RNA（mRNA）数据库，针对45日龄与75日龄组间比较，预测了lncRNA-miRNA、miRNA-mRNA及lncRNA-mRNA互作调控对，最终获得516对lncRNA-mRNA、1011对lncRNA-miRNA及7411对miRNA-mRNA调控关系对。随后，本研究对靶基因进行基因本体（Gene Ontology, GO）及京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析，结果显示靶基因主要富集于与毛囊发育密切相关的Wnt信号通路及PI3K-Akt信号通路，提示lncRNA可通过与miRNA/mRNA相互作用，直接或间接调控毛囊发育相关基因的表达。双荧光素酶报告基因分析证实，lncRNA MSTRG.1705.1可与Chi-miR-1结合，而lncRNA MSTRG.11809.1未发现与Chi-miR-433的结合位点。综上，本研究旨在进一步解析毛囊发育的分子调控机制，为揭示内蒙古绒山羊绒毛囊生长的调控机理奠定理论基础。