Homo sapiens Raw sequence reads. Homo sapiens

In this study, we developed a reliable FACS-based procedure using ethanol as a fixative that would support accurate isolation of limbal epithelial progenitor subpopulations along with RNA yield and quality sufficient to enable deep transcriptomic profiling.

本研究开发了一种以乙醇作为固定剂的可靠荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）流程，可精准分离角膜缘上皮祖细胞亚群，同时获得足以支持深度转录组分析的RNA产量与质量。