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Data underlying the publication: Mapping recombination landscape and basidial spore number in the button mushroom Agaricus bisporus

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Figshare2021-05-28 更新2026-04-28 收录
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https://figshare.com/articles/dataset/Data_underlying_the_publication_Mapping_recombination_landscape_and_basidial_spore_number_in_the_button_mushroom_Agaricus_bisporus/14695425
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- Raw data of counts of basidial spore numbers in lamellae of 2 varieties of A. bisporus, i.e. var. bisporus and var. burnettii (parents).The intervarietal hybrid and the intercross of haploid offspring of the intervarietal hybrid. These data sets are used to map QTL of the basidial spore number.- A selection of 71 haploid offspring of HBT03 that was outcrossed with H39. This set was used to generate 40-50 haploid offspring that was subsequently genotyped to assess crossover positions.These data were used to QTL map the trait " recombination landscape"- Genotypes of the intervarietal hybrid HBT03 (var. bisporus H97 x var. burnettii H119p4).180 homokaryotic single spore isolates (SSIs) were genotyped using 215 SNP's using Kasp genotyping method.- 71 haploid offspring of the intervarietal hybrid HBT03 were outcrossed with the var. bisporis homokaryon H39.From each hybrid, 40-50 haploid offspring was genotyped using 4 SNP markers per chromosome:markers at the chromosome ends, and 2 markers 150-200 kb from chromosome ends ("border" marker).

- 本数据集包含双孢蘑菇(Agaricus bisporus,简称A. bisporus)两个变种的菌褶担孢子数量计数原始数据,即作为亲本的原变种双孢蘑菇(var. bisporus)与伯内特双孢蘑菇(var. burnettii);同时涵盖该两变种的种间杂交菌株,以及该种间杂交菌株单倍体后代的互交群体数据。本数据集用于绘制担孢子数量的数量性状位点(Quantitative Trait Locus,QTL)图谱。 - 选取71株HBT03与H39杂交获得的单倍体后代。该群体用于衍生40~50株单倍体子代,随后对这些子代进行基因型分型以评估染色体交换位点。本数据集用于绘制“重组景观”性状的数量性状位点图谱。 - 种间杂交菌株HBT03(由原变种双孢蘑菇H97与伯内特双孢蘑菇H119p4杂交获得)的基因型数据。采用Kasp基因分型技术,利用215个单核苷酸多态性(Single Nucleotide Polymorphism,SNP)标记,对180株同核体单孢子分离株(Single Spore Isolates,SSIs)进行基因型分型。 - 将71株种间杂交菌株HBT03的单倍体后代与双孢蘑菇变种(var. bisporis)同核体菌株H39进行异型杂交。从每个杂交后代中选取40~50株单倍体后代,利用每条染色体上的4个单核苷酸多态性标记进行基因型分型:分别为位于染色体末端的标记,以及距离染色体末端150~200kb的2个标记(即“边界标记”)。
创建时间:
2021-05-28
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