DNA methylation in mouse liver tissue

We performed affinity-based enrichment with methyl-CpG binding domain protein followed by high-throughput sequencing (MBD-seq) to assay DNA methylation in mouse liver tissue. Mouse tissue were from two different inbred strains (C57BL/6J and DBA/2J) and from 3 age groups classified as young (4 months), mid (11–12 months) and old (24 months).

我们通过甲基-CpG结合域蛋白（methyl-CpG binding domain protein）介导的亲和富集结合高通量测序（MBD-seq）的方法，对小鼠肝脏组织的DNA甲基化水平进行检测。本研究的小鼠组织来自两个不同的近交系品系（C57BL/6J与DBA/2J），并分为三个年龄组别：青年组（4月龄）、中年组（11~12月龄）以及老年组（24月龄）。