Vitis riparia shoot tip transcriptome in response to long and short photoperiod.

Winter season with reduced day length (photoperiod); led to the growth cessation, dormancy induction and cold acclimation in woody perennial plants. To develop an understanding of the photoperiod signal transduction in Vitis riparia; shoot tip transcriptome profiling was performed under differential photoperiod treatments (long (LD, 15h) and short day (SD, 13h)) for 7 or 21 days after shoots reached 10 nodes (LD7, SD7, LD21 or SD21). Overall design: Three biological replicates of LD and SD shoot tip RNA were sequenced and analysed.

冬季昼长（光周期）缩短可诱导多年生木本植物出现生长停止、休眠诱导与冷驯化过程。为解析河岸葡萄（Vitis riparia）的光周期信号转导机制，本研究在枝条长至10个节位后，分别施以长日照（LD, 15h）与短日照（SD, 13h）处理，处理时长为7天或21天，随后对枝条顶端开展转录组表达谱分析，对应分组为LD7、SD7、LD21与SD21。实验整体设计：针对长日照与短日照处理的枝条顶端RNA，设置3次生物学重复并进行测序与数据分析。