RNA sequencing analyses of grl/hey2-overexpressing hearts and control hearts, as well as grl/hey2-deficient hearts and wild-type hearts following ventricular resection at 7 dpa

As Grl/Hey2 directly binds DNA through E box motifs and mediates transcription repression, we aim to gain insights into potential target genes of Grl/Hey2 during heart regeneration. We performed RNA-seq analyses using total RNAs collected from 4-HT-treated Tg(cmlc2:creER;cmlc2:nRSGG) hearts and Tg(cmlc2:nRSGG) control hearts, as well as grl5nt-/- mutant hearts and wild-type hearts following ventricular resection at 7 dpa. Using RNA profile analyses, we have identified some potential genes act downstream of Grl/Hey2-dependent transcriptional repression during heart regeneration.

鉴于Grl/Hey2可通过E盒基序（E box motifs）直接结合DNA并介导转录抑制，本研究旨在解析心脏再生过程中Grl/Hey2的潜在靶基因。我们收集了经4-HT处理的Tg(cmlc2:creER;cmlc2:nRSGG)心脏、Tg(cmlc2:nRSGG)对照心脏，以及心室切除术后7天（7 dpa）的grl5nt-/-突变体心脏与野生型心脏的总RNA，开展了RNA测序（RNA-seq）分析。通过RNA表达谱分析，我们已鉴定出若干在心脏再生过程中受Grl/Hey2依赖型转录抑制调控的潜在下游基因。