ChIP-Seq analysis of V5-tagged budding yeast Rif1

Genomic locations of V5-tagged budding yeast Rif1 (including wilt-type and designer mutants) were analysed by ChIP-Seq. Mutants tested were rif1-7A and rif1-7E, in which Ser/Thr residues in the cluster of SQ/TQ sites were mutated to Ala or Glu, respectively. We also tested tested rif1-?594, in which the C-terminal 594 amino acids were deleted.

本研究通过染色质免疫沉淀测序（ChIP-Seq）技术，分析了带V5标签的出芽酵母Rif1的基因组定位，样本涵盖野生型（wild-type）与定制突变体。本次测试的突变体包括rif1-7A与rif1-7E：二者SQ/TQ位点簇内的丝氨酸/苏氨酸（Ser/Thr）残基分别被突变为丙氨酸（Ala）或谷氨酸（Glu）；此外还测试了rif1-Δ594突变体，该突变体的C端594个氨基酸被截除。