GuHCl-induced unfolding of L35Ae samples
收藏Figshare2016-01-19 更新2026-04-08 收录
下载链接：
https://figshare.com/articles/dataset/GuHCl_induced_unfolding_of_L35Ae_samples/1465019/1
下载链接
链接失效反馈官方服务：
资源简介：
GuHCl-induced unfolding of L35Ae samples followed by fluorescence intensity at 314 nm and position of tryptophan fluorescence spectrum maximum. Excitation wavelength was 280 nm. Protein concentration was 3 µM. Buffer conditions: PBS, 150 mM NaCl, pH 7.0. Boltzmann function was used for theoretical fits of the experimental data.
采用盐酸胍（GuHCl）诱导L35Ae样品发生解折叠，通过检测314 nm处的荧光强度以及色氨酸荧光光谱的峰值位置对该过程进行追踪监测。实验激发波长设定为280 nm，蛋白浓度为3 μM。缓冲液条件为：磷酸盐缓冲液（PBS），含150 mM氯化钠，pH值7.0。实验数据的理论拟合采用玻尔兹曼函数完成。
创建时间：
2015-06-29
