Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Secondary screening for 20mGyS familly).. Mus musculus

Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. C57BL/6JNrs male mice were maintained in non-irradiated room for 400 days from 8 weeks of age. This study investigated the De novo mutation by comparing the parents and children. This is secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan. Overall design: Two-condition experiment, C57 BL/6JNrs tail tissue non-irradiated male (20mGySM), female (20mGySF) and their progenies (20mGyS1 to 20mGyS3) vs. reference male mouse.

持续低剂量率（low dose-rate, LDR）γ射线照射的跨代效应尚未得到充分研究。本研究以C57BL/6JNrs雄性小鼠为对象，自8周龄起于无辐照饲养环境中饲养400天。本研究通过比较亲代与子代样本，对新生突变（de novo mutation）展开探究。本次实验属于二次筛选。本研究受日本青森县政府委托开展。整体实验设计：采用双组对照实验，将未辐照的C57BL/6JNrs雄性小鼠（20mGySM）、雌性小鼠（20mGySF）及其子代（20mGyS1至20mGyS3）的尾部组织与对照雄性小鼠进行对比分析。