SH1039320.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE提供了一套统一的标准化流程，用于界定、识别、交流并处理基于DNA的物种假说（Species Hypotheses，以下简称SH）。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一系列动态距离阈值（0.5%至3.0%）被聚类至近似物种层级。所有物种假说均会被赋予唯一且稳定的数字对象唯一标识符（Digital Object Identifier，DOI）格式的专属名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作完成校验。系统会结合每个SH下所有序列的鉴定结果，尽可能将其匹配至对应的分类单元名称及其分类层级（如门、纲、目等）。将为每个SH选取一条经自动或人工指定的代表序列。上述代表序列已公开发布于（https://unite.ut.ee/repository.php），可供科研社区用于本地序列相似性搜索、高通量测序分析流程等场景。随着公共真菌ITS序列数量持续增长，该系统及其配套数据将自动完成更新。