Estimating somatic mutation rates by Duplex Sequencing in non-model organisms: Daphnia magna as a case study

Duplex sequencing was performed on five bottlenecked WGS libraries (10, 50, 100, 150, or 1000 amol of input DNA) of Daphnia magna to estimate somatic mutation rates.

研究人员对5个来自大型溞（Daphnia magna）、经瓶颈化处理的全基因组测序（Whole Genome Sequencing, WGS）文库（输入DNA量分别为10、50、100、150或1000阿托摩尔（amol））开展双工测序（Duplex sequencing），以估算其体细胞突变率。