Methylated CpG Island Recovery Assay (MIRA)-chip from Sirt1 wild-type (Sirt1+/+) and knock-out (Sirt1-/-) murine embryonic stem cells (ESCs)

Proper DNA-methylation is inevitable for normal development and this epigenetic program is dynamically reprogrammed especially around embryo implantation. Accordingly, embryonic stem cells (ESCs) derived from inner cell mass in pre-implantation embryo exhibit the lowest DNA methylation level compared with differentiated tissue cells to ensure their pluripotency and germ-line potency. Methylated CpG Island Recovery Assay (MIRA) coupled chip DNA methylome analysis demonstrated that Sirt1 deficiency increases the abnormal DNA methylation especially on the a subtype of imprinted and germ-line development genes. Comparison of DNA methylome between Sirt1+/+ and Sirt1-/- murine ESCs

正常发育过程中，恰当的DNA甲基化不可或缺，这一表观遗传程序尤其在胚胎着床前后会发生动态重编程。据此，从着床前胚胎内细胞团分离得到的胚胎干细胞（embryonic stem cells, ESCs），与分化组织细胞相比，其DNA甲基化水平最低，以此维持自身的多能性与生殖系潜能。采用甲基化CpG岛回收检测（Methylated CpG Island Recovery Assay, MIRA）联合芯片DNA甲基化组分析的结果显示，沉默信息调节因子1（Sirt1）缺失会加剧异常DNA甲基化，尤其在印记基因与生殖系发育基因的某一亚型上。对Sirt1+/+与Sirt1-/-小鼠胚胎干细胞的DNA甲基化组进行比较分析。