BETA-FOR_SP9_Biomass_Malaise_2022-2025

one-time sampling 2022 + rapid assessment 2023 - 2025 ################ This dataset includes records of insect biomass collected using Malaise traps across the 234 BETA-FOR patches. One Malaise trap was installed on each patch near the patch centre. Traps followed the Towns design (front height: 0.90 m; rear height: 0.60 m; length: 1.60 m) with black mesh. Trapping was conducted from May to July. Traps were emptied twice per month (mid-month and end of month), resulting in up to six samples per patch and year. Sampling was carried out in 2022 for 90 patches in the University Forest and 90 patches in the Bavarian Forest and Passau regions. In 2023, the 54 patches located in Saarland, Hunsrück, and Lübeck were sampled. From 2023 onwards until 2025, sampling continued only on the rapid assessment patches located in the University Forest, Bavarian Forest, and Passau regions. Collected samples were preserved in 70% ethanol. Prior to metabarcoding, samples were separated into large and small fractions using an 8 mm sieve to reduce biomass bias from larger specimens. Samples were drained and the wet biomass of the small and large fractions was weighed separately using a laboratory balance. The resulting fractions were subsequently stored in 90% ethanol. For analyses of total insect biomass, the column "wetBiomass_total" should be used. Missing wet biomass values were estimated based on the "dryBiomass" measurement, which represents the weight of the sample after drying prior to DNA extraction. ################ We greatly acknowledge the help of: Jérôme Morinière (AIM/Metabarcoding), Linda Seifert, Michael Junginger, Elisa Stengel, Christopher Geus, Katharina Kallnik, Nina Wild, Fof Brixy, Manuel Mauermann, Kim Zimmermann, Noelle Demuth, Adèle Kieffer, Soumen Mallick, Mareike Kortmann, Interns of the Bavarian Forest Nationalpark and all other assistants2022年一次性采样 + 2023-2025年快速评估 本数据集涵盖通过马氏陷阱（Malaise trap）在234个BETA-FOR样地采集的昆虫生物量记录。每个样地的中心附近均布设1台马氏陷阱。陷阱采用汤氏设计（Towns design），配备黑色网纱，参数为：正面高度0.90 m、背面高度0.60 m、长度1.60 m。 采样周期为5月至7月。每月对陷阱进行两次清空（分别于月中与月末），每个样地每年最多可获取6份样品。 2022年，我们对大学林区的90个样地以及巴伐利亚林区、帕绍地区的90个样地完成采样；2023年，对萨尔兰州、洪斯吕克地区与吕贝克的54个样地开展采样；2023年起至2025年，仅对大学林区、巴伐利亚林区及帕绍地区的快速评估样地持续进行采样。 采集得到的样品保存于70%乙醇中。在开展元条形码测序（metabarcoding）前，使用孔径8 mm的筛网将样品分为大、小两个组分，以降低大型标本带来的生物量偏差。随后沥干样品，分别使用实验室天平称量大、小组分的鲜生物量。处理后的组分最终保存于90%乙醇中。 若需分析昆虫总生物量，应使用"wetBiomass_total"列。缺失的鲜生物量值可通过"dryBiomass"测量值估算，该参数代表DNA提取前样品干燥后的重量。 我们衷心感谢以下人员的协助：Jérôme Morinière（AIM/元条形码测序团队）、Linda Seifert、Michael Junginger、Elisa Stengel、Christopher Geus、Katharina Kallnik、Nina Wild、Fof Brixy、Manuel Mauermann、Kim Zimmermann、Noelle Demuth、Adèle Kieffer、Soumen Mallick、Mareike Kortmann、巴伐利亚林区国家公园实习生及所有其他协助人员