Transcriptome analysis of alternative splicing under heat stress condition in atcyp18-1 mutant compared to wild type of Arabidopsis

To investigate whether the observed changes in pre-mRNA splicing led to AtCYP18-1 depletion, we conducted RNA-sequencing to analyse the quantitative profiling of alternative splicing Overall design: RNA was immediately extracted from atcyp18-1 and WT seeds and treated with or without heat stress for 2 h at 1 day after imbibition.

为探究观测到的前体mRNA（pre-mRNA）剪接变化是否导致AtCYP18-1的缺失，我们开展RNA测序（RNA-sequencing）以分析可变剪接的定量谱图。
整体实验设计：在种子吸胀1天后，即刻从atcyp18-1突变体与野生型（WT）种子中提取RNA，随后分别施加或不施加热胁迫处理，持续2小时。