Data_Sheet_1_Efficacy of Tigecycline and Linezolid Against Pan-Drug-Resistant Bacteria Isolated From Companion Dogs in South Korea.XLSX

The emergence of multidrug-resistant bacteria in companion animals is an increasing concern in view of the concept of One Health. The antimicrobials linezolid (LZD) and tigecycline (TGC) are effective against multidrug-resistant bacteria isolated from humans; however, thus far, no previous study has evaluated the efficacy of these drugs against bacteria isolated from companion animals. This study aimed to evaluate the efficacy of LZD and TGC against bacteria that were isolated from companion dogs and showed resistance to all classes of antimicrobial agents. Clinical samples (auditory channel, eye, skin, and urine) were collected from dogs that visited the Veterinary Medical Teaching Hospital of Konkuk University (Seoul, South Korea) from October 2017 to September 2020. In total, 392 bacterial isolates were obtained, of which 85 were resistant to all classes of antimicrobial agents tested and were, therefore, considered potentially pan-drug resistant (PDR). The susceptibility of isolates to LZD and TGC was determined by the disk diffusion method and interpreted using the Clinical Laboratory Standards Institute guidelines. In total, 95.6% (43/45) and 97.8% (44/45) of gram-positive isolates were susceptible to LZD and TGC, respectively, whereas 82.5% (33/40) of gram-negative isolates were sensitive to TGC. In conclusion, both agents showed favorable efficacy, with the susceptibility rates for all potential PDR bacteria, except Pseudomonas spp., ranging from 72.7 to 100%. Thus, these drugs may serve as excellent antimicrobial options for veterinary medicine in the future.

鉴于同一健康（One Health）理念，伴侣动物体内多重耐药菌的出现已成为日益受到关注的问题。利奈唑胺（linezolid, LZD）与替加环素（tigecycline, TGC）均为抗人类分离多重耐药菌的有效抗菌药物，但截至目前，尚无研究评估这两类药物对伴侣动物分离细菌的抗菌活性。本研究旨在评估利奈唑胺与替加环素对从伴侣犬分离得到的、对所有类别抗菌药物均耐药的细菌的抗菌效果。本研究于2017年10月至2020年9月期间，从韩国首尔建国大学兽医学教学医院就诊的犬只身上采集临床样本（耳道、眼部、皮肤及尿液样本），最终共分离得到392株细菌，其中85株对本次试验所用的全部类别抗菌药物均耐药，因此被判定为潜在泛耐药（PDR）菌株。采用纸片扩散法（disk diffusion method）测定分离菌株对利奈唑胺与替加环素的药敏性，并依据临床实验室标准协会（Clinical Laboratory Standards Institute, CLSI）指南进行结果判读。结果显示，革兰氏阳性分离菌株中，分别有95.6%（43/45）与97.8%（44/45）对利奈唑胺、替加环素敏感；而革兰氏阴性分离菌株中有82.5%（33/40）对替加环素敏感。综上，两种药物均展现出良好的抗菌活性：除假单胞菌属（Pseudomonas spp.）菌株外，所有潜在泛耐药菌株的药敏率介于72.7%至100%之间。因此，这两类药物未来有望成为兽医学领域的优质抗菌药物选择。