Arbuscular mycorrhizal fungal community rRNA gene sequences from prairie restoration experiment. rhizosphere metagenome

These sequences are from a prairie restoration experiments testing the combined effects of two soil amendments: arbuscular mycorrhizal (AM) fungi strains from remnant prairie sites (biotic soil amendment), and biochar (carbonized organic matter, an abiotic soil amendment) on the AM fungal community in the restoration plots. Sequences are derived from the AM fungi that colonized plant roots collected from soil samples taken in each of the first two years of the restoration experiment. The PCR primers used amplify a portion of the large subunit (28S/LSU) nuclear ribosomal RNA gene, and are fairly specific to AM fungi although they also amplify sequences from other fungi. Sequences are paired 300bp reads from an Illumina MiSeq instrument.

本数据集的序列源自一项草原修复试验，该试验旨在评估两种土壤改良剂（soil amendment）对修复样地内丛枝菌根（arbuscular mycorrhizal, AM）真菌群落的联合效应：其一为采自残存草原样地的AM真菌菌株，属于生物土壤改良剂（biotic soil amendment）；其二为生物炭（biochar，即碳化有机质，属于非生物土壤改良剂abiotic soil amendment）。本数据集的序列来源于该修复试验开展前两年各采样点采集的土壤样本中，定殖于植物根系的AM真菌。所用PCR引物可扩增核核糖体RNA基因（nuclear ribosomal RNA gene）的大亚基（28S/LSU）片段，该引物对AM真菌具有较高特异性，但同时也能扩增其他真菌的序列。本数据集的序列为Illumina MiSeq测序平台产出的300bp双端测序读段。