Data on environmental DNA detection of Medionidus simpsonianus in the Ochlockonee River (June 2021/December 2022)

Data are associated with detection of Medionidus simpsonianus in the Ochlockonee River using tactile surveys and a newly developed single-species environmental DNA (eDNA) assay tested on water samples. Data include a NADH dehydrogenase subunit 1 alignment that was used to develop primer and probe components and test target species specificity in situ, sample data from field collection and laboratory processing, and droplet digital Polymerase Chain Reaction (ddPCR) results for field samples, controls, and experiments of spin column purification effects on eDNA yield and PCR inhibitor removal. We also provide all known Medionidus simpsonianus occurrence records as of September 2023.

本数据集涵盖了在奥克拉霍尼河（Ochlockonee River）中，通过触觉调查与基于水样开发的新型单物种环境DNA（environmental DNA, eDNA）检测方法，开展Medionidus simpsonianus探测的相关数据。数据集包含以下内容：用于开发引物与探针组件、原位验证靶标物种特异性的烟酰胺腺嘌呤二核苷酸脱氢酶亚基1（NADH dehydrogenase subunit 1）序列比对数据；野外采集与实验室处理流程对应的样本数据；针对野外样本、对照样本的液滴数字聚合酶链式反应（droplet digital Polymerase Chain Reaction, ddPCR）结果，以及关于离心柱纯化法对eDNA回收率与PCR抑制剂去除效果的实验数据。此外，本数据集还提供了截至2023年9月的所有已知Medionidus simpsonianus出现记录。