MIC genomes: Sequencing data and size estimates.
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(a) Highly enriched sorted MIC (97%–99%) are contaminated with the highly polyploid MAC. For example, 97% of MIC purity led to 60% of MAC DNA contamination in the case of P. tetraurelia (see S2 Fig). MIC genome size (in Mb) was estimated based on (b) flow cytometry analysis and (c) k-mer counts. Size estimation before correction, based on MAC contamination, is indicated in parentheses (see Materials and methods). MAC, macronucleus; MIC, micronucleus. (XLSX)
(a) 经分选得到的高富集小核（micronucleus, MIC）纯度为97%~99%，但会被多倍体程度极高的大核（macronucleus, MAC）污染。例如在P. tetraurelia案例中，当小核纯度为97%时，大核DNA污染占比可达60%（详见补充图2，S2 Fig）。 (b) 小核（micronucleus, MIC）的基因组大小（单位：兆碱基，Mb）通过(b) 流式细胞术分析与(c) k-mer计数两种方式估算。 基于大核（macronucleus, MAC）污染情况的校正前基因组大小估算值已标注于括号内（详见材料与方法）。 注：MAC即大核（macronucleus），MIC即小核（micronucleus）。(XLSX)
