Regulatory elements coordinating initiation of chromosome replication to the Escherichia coli cell cycle

Bacteria replicate the genome on average once per generation, irrespective of growth condition-dependent changes in generation time and cell size. The strategy appears to be to initiate replication in a narrow chromosome-to-size ratio interval for each growth condition. To investigate how Escherichia coli accurately compares its size to the number of chromosomes, we perturbed some of the mechanisms proposed to achieve this precision and monitored how replication initiation was affected with time-lapse single-cell fluorescence microscopy. We found that the regulatory inactivation of DnaA-ATP (RIDA) mechanism, which converts the replication initiation molecule DnaA to its inactive form in proportion to the number of chromosomes, was the most important factor and that other previously described mechanisms have a less critical role. 

This dataset contains the raw data, analysis and code needed to generate the figures presented in the paper. The raw data consists of microscopy images, growth rate measurements, RT-qPCR, whole-genome sequencing and Rif-runout data. The analysis and code part consists of code and output from the analysis and post-processing.

细菌平均每一代复制一次基因组，不受生长条件依赖的代时与细胞大小变化的影响。该类细菌的复制策略似乎为：针对每种生长条件，在狭窄的染色体-细胞大小比例区间内启动复制。为探究大肠杆菌（Escherichia coli）如何精准比对其细胞大小与染色体数目，我们对已提出的若干实现该精准调控的机制进行了扰动，并通过延时单细胞荧光显微镜监测复制起始过程所受影响。研究发现，DnaA-ATP调控失活（RIDA）机制——该机制可按染色体数目比例将复制起始分子DnaA转化为非活性形式——是最为关键的调控因子，而此前报道的其他机制所起作用相对次要。

本数据集包含生成论文中所有图表所需的原始数据、分析脚本与代码。原始数据涵盖显微镜图像、生长速率测定结果、实时定量PCR（RT-qPCR）数据、全基因组测序（whole-genome sequencing）数据以及利福平耗尽（Rif-runout）实验数据；分析与代码部分则包含分析及后处理过程所使用的代码与输出结果。