Deep genomic divergence and phenotypic admixture of the treefrog Dendropsophus elegans (Hylidae: Amphibia) coincide with riverine boundaries at the Brazilian Atlantic Forest

Here, we sample genome-wide RADseq data from a common, widespread tree frog (Dendropsophus elegans) inhabiting natural and human-impacted ecosystems at the Brazilian Atlantic Forest biome to test the impact of riverine boundaries and climatic refugia on population structure and diversification. We estimate divergence times and migration across identified genetic breaks related to the rivers Doce, Paraiba do Sul, Ribeira, and Paraguacu, known to represent barriers to gene flow for other Atlantic Forest endemic species, and test the role of climatic refugia. Finally, we investigate the impact of spatio-temporal population history on morphological variation in this species.

本研究采集栖息于巴西大西洋森林生物群区自然与受人类活动干扰生态系统中的广布常见树蛙——优雅雨蛙（Dendropsophus elegans）的全基因组RADseq（限制性位点相关DNA测序，Restriction-site Associated DNA sequencing）数据，以检验河流边界与气候避难所对种群结构及演化分化的影响。我们估算了与多西河（Doce）、南帕拉伊巴河（Paraiba do Sul）、里贝拉河（Ribeira）及帕拉瓜苏河（Paraguacu）相关的已鉴定遗传断裂带的分化时间与基因流水平；上述河流已被证实可作为其他大西洋森林特有物种的基因流屏障，本研究同时检验了气候避难所的作用。最后，我们探讨了时空种群历史对该物种形态变异的影响。