Quantitative multiplexed ChIP-Seq comparing mouse embryonic stem cells grown in Serum or 2i condition.. Quantitative multiplexed ChIP-Seq comparing mouse embryonic stem cells grown in Serum or 2i condition.

Quantitative multiplexed ChIP-Seq reveals high levels of H3K27me3 broadly covering the genome and low levels of H3K4me3 in 2i condition (ground state). Bivalent promoters, a defining feature of pluripotent cells, are also present in the ground state. H3K27me3 is maintained at high levels at bivalent promoters, similar to those in Serum, while H3K4me3 is reduced more than twofold. Overall design: Rw4 mouse embryonic stem cells were grown in either serum-free medium with PD0325901 and CHIR99021 (“2i”), regular serum-based medium (“Serum”), or serum-based medium with 2i (“2i/Serum”) for at least three passages. mTORi cells were treated with 200nM INK128 (VWR, CAYM11811) for 48h. 1e6 cells of each condition/replicate were collected. This dataset contains MINUTE-ChIP data from three pools (P1-3). Biological replicates, where applicable, are stored in distinct raw files under the same entry and combined in the processed data file

定量多重染色质免疫共沉淀测序（Quantitative multiplexed ChIP-Seq）结果显示，在2i条件（基态，ground state）下，组蛋白H3第27位赖氨酸三甲基化（H3K27me3）广泛覆盖全基因组且丰度较高，而组蛋白H3第4位赖氨酸三甲基化（H3K4me3）丰度较低。二价启动子（bivalent promoters）作为多能细胞的标志性特征，同样存在于该基态中。H3K27me3在二价启动子处仍维持高丰度，与血清培养条件下的水平相近，而H3K4me3的丰度则下调两倍以上。实验设计：将Rw4小鼠胚胎干细胞（mouse embryonic stem cells）分别在三种培养体系中传代至少3次：添加PD0325901与CHIR99021的无血清培养基（"2i"组）、常规血清培养基（"Serum"组），以及添加2i的血清培养基（"2i/Serum"组）。mTORi细胞采用200nM的INK128（VWR, CAYM11811）处理48小时。每个培养条件/生物学重复组收集1×10^6个细胞。本数据集包含来自三个混合样本（P1-P3）的MINUTE-ChIP数据。若存在生物学重复，重复样本将以独立原始文件存储于同一条目下，并在处理后的数据文件中进行合并。