Heterologous expression analysis of wild type Fusarium fujikuroi and Ffvel1deletion strains grown under nitrogen-limiting conditions in liquid media over time.. Fusarium fujikuroi

Investigation of whole genome gene expression level differences of Fusarium fujikuroi between wild-type and a Ffvel1 (velvet) deletion mutant in liquid medium with minimal nitrogen between 24 hr, 72 hr and 120 hr of growth using an array based on a F. verticillioides gene call set. Fusarium fujikuroi produces a number of secondary metabolites including gibberellins, bikaverin, fumonisin and fusarin C that are influenced by nitrogen availability and the velvet global regulatory complex. Overall design: A twelve chip study using total RNA recovered from six cultures of wild-type Fusarium fujikuroi and six cultures of Ffvel1 F. fujikuroi deletion mutant. Each chip measures the expression level of over 13,000 putative genes with twelve 60-mer probes per sequence.

本研究采用基于轮枝镰孢（Fusarium verticillioides）基因预测集（gene call set）构建的基因芯片，针对在低氮液体培养基中分别生长24小时、72小时及120小时的野生型藤仓镰孢（Fusarium fujikuroi）与Ffvel1（绒状调控复合体，velvet）缺失突变体，开展全基因组基因表达水平差异分析。藤仓镰孢可合成多种次级代谢产物，包括赤霉素（gibberellins）、比卡维林（bikaverin）、伏马菌素（fumonisin）以及镰孢菌素C（fusarin C），上述产物的合成均受氮素可利用性及绒状全局调控复合体（velvet global regulatory complex）的调控。实验整体设计：本研究共使用12张芯片，样本来源于6份野生型藤仓镰孢培养物与6份Ffvel1缺失突变体藤仓镰孢培养物的总RNA。每张芯片可检测超过13000个推定基因的表达水平，每个基因序列对应12条60聚体寡核苷酸探针（60-mer probes）。