Profiling of protrusion-enriched RNAs from human breast cancer cell line MDA-MB-231

The goals of this study are to compare the gene expression of cell protrusions and corresponding cell bodies by isolating cell protrusions from cultured MDA-MB-231 cells in a novel microfluidic device. Overall design: mRNA profiles of cell protrusions and corresponding cell bodies of MDA-MB-231 cells were gnertated by deep sequencing, in five replicates, using Illumina Hiseq 4000.本研究旨在通过在新型微流控装置（microfluidic device）中从培养的MDA-MB-231细胞中分离细胞突起（cell protrusions），对比细胞突起与其对应细胞体的基因表达水平。 实验设计概述：MDA-MB-231细胞的细胞突起及其对应细胞体的mRNA转录组谱通过Illumina HiSeq 4000平台进行深度测序生成，共设置5次重复。