MicroRNA-198 an inhibitor of keratinocyte migration. Homo sapiens

This study identifies miR-198 as a potential inhibitor of keratinocyte migration in skin This study identifies genes differentially expressed during the early phases of wound healing using an ex vivo organ culture model of human skin Overall design: Total RNA from N/TERT-1 keratinocytes transfected with a negative control miRNA or miR-198 were subjected to microarray analysis Skin from elective plastic surgery were subjected to wounding using ex vivo organ culture model. Total RNA from skin biopsies were isolated at 0hr and 24hr post injury and subjected to mircoarray analysis

本研究鉴定miR-198为皮肤角质形成细胞迁移的潜在抑制剂，并利用人体皮肤离体器官培养模型，筛选伤口愈合早期阶段的差异表达基因。实验设计：将转染阴性对照miRNA或miR-198的N/TERT-1角质形成细胞的总RNA进行微阵列（microarray）分析；另取择期整形外科手术获取的皮肤组织，通过离体器官培养模型实施创伤造模，分别于创伤后0小时与24小时提取皮肤活检组织的总RNA，同样开展微阵列分析。