DDX24 Promotes Lymphangiogenesis and Lymph Node Metastasis via AGRN Production in Cervical Squamous Cell Carcinoma

To investigate the factor produced by CSCC cells to facilitate lymphangiogenesis, we analyzed the transcriptomics of CSCC cells upon DDX24 has been knocked down by siRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cell lines. Comparative gene expression profiling analysis of RNA-seq data for Cervical Squamous Cell Carcinoma cell lines (MS751 and SiHa) and their DDX24 KD derivatives .

为探究宫颈鳞状细胞癌（Cervical Squamous Cell Carcinoma, CSCC）细胞分泌的促淋巴管生成因子，我们通过小干扰RNA（small interfering RNA, siRNA）敲低CSCC细胞内的DDX24基因，随后对该敲低处理后的CSCC细胞开展转录组分析。后续我们利用两种不同细胞系的RNA测序（RNA-seq）数据进行基因表达谱分析，并针对宫颈鳞状细胞癌（CSCC）细胞系MS751、SiHa及其DDX24敲低（knockdown, KD）衍生细胞系的RNA-seq数据开展对比基因表达谱分析。