Characterization of novel natural compound derivatives with cancer-selective cytotoxicity (RNA-seq dataset 3)

Colorectal cancer is one of the most frequent and lethal cancer in the world. Current therapeutic approaches are still not fully successful, yet cause numerous and severe side effects for the patient. We screened chemically synthesized derivatives from two natural compounds (a-Mangostin and Paeonol) as potential novel chemicals that increase cancer cell selectivity over non-transformed human cells. We found two of them that efficiently induced cell cycle arrest and apoptosis in human cancer cells and organoids derived from tumor tissue without affecting the viability of human non-cancer cells and intestinal organoids derived from healthy tissue. Our findings increase the knowledge about cancer-specific compounds and propose two natural compound derivatives as potential novel chemotherapeutics. Overall design: Transcriptional profile of colon cancer cell lines upon treatment of two cancer-selective novel natural compounds

结直肠癌（Colorectal cancer）是全球范围内最常见且致死率最高的恶性肿瘤之一。当前的临床治疗方案尚未能完全取得理想疗效，且会给患者带来诸多严重的不良反应。我们从两种天然化合物——α-倒捻子素（α-Mangostin）与丹皮酚（Paeonol）——的化学合成衍生物中，筛选出了可提升肿瘤细胞相较于非转化人类细胞选择性的潜在新型化合物。研究发现，其中两种化合物可在人类肿瘤细胞及肿瘤组织来源的类器官（organoids）中高效诱导细胞周期阻滞（cell cycle arrest）与细胞凋亡（apoptosis），且不会影响人类非肿瘤细胞以及健康组织来源的肠道类器官的活性。本研究的发现加深了学界对肿瘤特异性化合物的认知，并提出两种天然化合物衍生物可作为潜在的新型化疗药物。实验整体设计：经两种肿瘤选择性新型天然化合物处理的结肠癌细胞系的转录组特征（Transcriptional profile）。