Rootstock induced scion resistance against tobacco mosaic virus is associated with the induction of defense related transcripts and graft transmissible mRNAs. Rootstock induced scion resistance against tobacco mosaic virus is associated with the induction of defense related transcripts and graft transmissible mRNAs

To investigate graft conferred resistance against viral diseases a novel hetero-grafting system was developed using Nicotiana benthamiana scions grafted onto different tomato rootstocks. RNAseq analysis was used to identify mobile tomato mRNAs within N. benthamiana scions Overall design: Mapping the scion derived transcripts to rootstock genome to identify mobile RNA

为探究嫁接赋予的抗病毒病害抗性，研究人员构建了一套新型异源嫁接体系：以本氏烟（Nicotiana benthamiana）接穗嫁接到不同番茄砧木上。通过RNA测序（RNAseq）分析，鉴定本氏烟接穗内的可移动番茄信使核糖核酸（mRNA）。整体实验设计：将接穗来源的转录本比对至砧木基因组，以鉴定可移动RNA。