Examining Pathogenesis in Genome Instability Associated Neurodegenerative Mouse Model [Total_PolII_Chip-seq]

Alterations in RNA Polymerase II kinetics can affect key co-transcriptional processes such as normal splicing and transcript elongation and can influence nonsense mediated decay (Fong et al., 2014; Jonkers and Lis, 2015). Specific types of DNA damage can variably influence RNA Pol II activity. We performed Total RNA Pol ll ChIP-seq to investigate how the chronic DNA damage can affect RNA Pol ll activity in the cerebellum tissues. Overall design: Chip- sequencing of the cerebellum looking at RNA polymerase II in ATM APTX null and associated control brains.

RNA聚合酶II（RNA Polymerase II）的动力学改变可影响关键共转录过程，例如正常剪接与转录延伸，还可调控无义介导降解（nonsense mediated decay）（Fong等人，2014；Jonkers与Lis，2015）。特定类型的DNA损伤会对RNA聚合酶II的活性产生差异化影响。本研究通过全RNA聚合酶II染色质免疫沉淀测序（Total RNA Pol II ChIP-seq），旨在探究慢性DNA损伤如何影响小脑组织中的RNA聚合酶II活性。整体实验设计：对小脑组织开展染色质免疫沉淀测序，以检测ATM APTX功能缺失型与配对对照脑组织中的RNA聚合酶II结合情况。