Molecular Characteristics of Cohorts Relative to Presence or Not of Chromosomal Instability (CIN).

CIN+ was indicated by the presence of LOH. This was scored for overall CIN+ if a cancer had 1 of the 12 markers showing greater than 40% loss in intensity of one allele compared to its paired normal. CIN- was assigned if 40% (5 of 12) markers were informative and none of these showed LOH. For individual regions, CIN+ was scored if 1 marker showed LOH, and CIN- if 2of the 3 markers were informative and neither had LOH. CIMP high was scored if 3 of the 5 markers were positive for methylation.
*CIN and corresponding p53 mutational status was analysed using CIN data at the 5q, 8p and 18q loci only.

染色体不稳定性阳性（CIN+）以杂合性缺失（LOH, Loss of Heterozygosity）的存在作为判定依据。针对整体样本的CIN+判定：若某癌症的12个检测标志物中，有1个标志物的某一等位基因相较于其配对正常样本的强度降低超过40%，则判定为CIN+。若12个标志物中有40%（即5个）可提供有效分析信息，且所有有效信息位点均未出现LOH，则判定为CIN-。针对单个检测区域：若存在1个标志物出现LOH，则判定为CIN+；若3个标志物中有2个可提供有效分析信息，且二者均未出现LOH，则判定为CIN-。高CpG岛甲基化表型（CIMP high）的判定标准为：5个检测标志物中有3个呈甲基化阳性。
*本次分析仅基于5q、8p及18q染色体位点的CIN数据，对CIN及对应p53突变状态进行了关联分析。