Genome-wide expression profile of livers from mice fed HFD,SO-HFD or Viv chow [RNAseq]. Mus musculus

Purpose: Aim of the study is to identify changes in hepatic gene expression induced by either a 40kcal% coconut oil rich high fat diet (HFD), a 40kcal% soybean oil plus coconut oil high fat diet (SO-HFD) or a low fat vivarium chow diet (Viv). Methods: Livers from mice that had been fed one of the above mentioned diets for 35 weeks, were used to make cDNA libraries that were then sent for deep sequencing, using the Illumina TruSeq RNA. Result: Many genes involved in metabolism, lipid binding, transport and storage and many Cyp genes are dysregulated in the two high fat diets as compared to Viv HFDs in SO-HFD mice. Comparing the two HFDs shows more metabolism and disease related genes dysregulated in SO-HFD vs HFD. Conclusion: A diet high in soybean oil may be more detrimental to metabolic health than a diet high in saturated fats. Overall design: cDNA isolated from livers from mice fed HFD, SO-HFD or Viv for 35 weeks, were 50bp pair-ended sequenced in triplicate using Illumina TruSeq RNA Sample Prep v2 Kit.

研究目的：本研究旨在明确分别饲喂40kcal%椰子油高脂饮食（High Fat Diet, HFD）、40kcal%大豆油联合椰子油高脂饮食（Soybean Oil plus Coconut Oil High Fat Diet, SO-HFD）以及低脂饲养室常规饲料（Vivarium Chow Diet, Viv）的小鼠，其肝脏基因表达的变化情况。
实验方法：将分别饲喂上述三种饮食35周的小鼠肝脏组织构建cDNA文库，随后采用Illumina TruSeq RNA测序平台开展深度测序。
实验结果：相较于低脂饲养室常规饲料组，两种高脂饮食组小鼠肝脏中均存在大量参与代谢、脂质结合、转运与储存的基因以及诸多Cyp基因表达失调，该现象在饲喂大豆油联合椰子油高脂饮食的小鼠中更为显著。对比两种高脂饮食组可见，相较于椰子油高脂饮食组，大豆油联合椰子油高脂饮食组中更多与代谢及疾病相关的基因出现表达失调。
研究结论：相较于富含饱和脂肪的高脂饮食，大豆油高脂饮食可能对代谢健康造成更为严重的损害。
整体实验设计：将分别饲喂高脂饮食、大豆油联合椰子油高脂饮食或低脂饲养室常规饲料35周的小鼠肝脏组织提取的cDNA，采用Illumina TruSeq RNA Sample Prep v2试剂盒构建文库，随后进行50bp双端测序，每组设置三次生物学重复。