CCR5_T30

To examine the signaling pathways active downstream ACKR2, both in constitutive and ligand-stimulated conditions, we carried out a large-scale mass spec-based quantitative phosphoproteomic analysis by SILAC technique. ACKR2 has been expressed in HEK293T cells using a tetracycline-inducible system and stimulated with the common ligand CCL3L1. Using computational approaches, we performed a comparative system-based analysis of the ACKR2- and CCR5-mediated phosphoproteomes.

为探究组成型与配体刺激条件下非典型趋化因子受体2（ACKR2）下游的激活信号通路，本研究采用稳定同位素标记氨基酸细胞培养（Stable Isotope Labeling by Amino acids in Cell culture, SILAC）技术，开展了大规模基于质谱（Mass Spectrometry, MS）的定量磷酸化蛋白质组学分析。我们通过四环素诱导表达系统在人类胚胎肾293T（HEK293T）细胞中过表达非典型趋化因子受体2（ACKR2），并以经典配体趋化因子配体3L1（CCL3L1）对细胞进行刺激。随后借助计算生物学方法，我们对非典型趋化因子受体2（ACKR2）与CC趋化因子受体5（CCR5）介导的磷酸化蛋白质组开展了系统层面的比较分析。