Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)

Sample collection:All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below. Metagenome sequencing and assembly:Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample.Description of ACE Sample IDs (including in filenames) and the relevant soil samples: *    SB9748-    Soil barcode= 91062-    Original barcode= 91604-    Sample Site= Browning Peninsula-    Transect= 3-    Distance samples= 2m-    Concentration of DNA sent= 1.2ng/µL (required concentration)-    Volume DNA sent= 80µL*    SB9749-    Soil barcode= 91604-    Original barcode= 26587-    Sample Site= Browning Peninsula-    Transect= 3-    Distance samples= 102m-    Concentration of DNA sent= 1ng/µL (required concentration)-    Volume DNA sent= 80µL*    SB9750-    Soil barcode= 91606-    Original barcode= 26617-    Sample Site= Browning Peninsula-    Transect= 3-    Distance samples= 202m-    Concentration of DNA sent= 0.3ng/µL (required concentration)-    Volume DNA sent= 180µL*    SB9751-    Soil barcode= 91447-    Original barcode= 36260-    Sample Site= Mitchell Peninsula-    Transect= 2-    Distance samples= 2m-    Concentration of DNA sent= 0.86ng/µL (required concentration)-    Volume DNA sent= 80µL*    SB9752-    Soil barcode= 91477-    Original barcode= 36290-    Sample Site= Mitchell Peninsula-    Transect= 2-    Distance samples= 102m-    Concentration of DNA sent= 0.9ng/µL (required concentration)-    Volume DNA sent= 80µL*    SB9753-    Soil barcode= 91507-    Original barcode= 36320-    Sample Site= Mitchell Peninsula-    Transect= 2-    Distance samples= 202m-    Concentration of DNA sent= 1.9ng/µL (required concentration)-    Volume DNA sent= 80µL

样本采集：所有土壤样本均采集于2005年，在采用FastDNA SPIN土壤基因组DNA提取试剂盒（FastDNA SPIN kit for soil，MP Biomedicals，澳大利亚新南威尔士州）进行基因组DNA（genomic DNA，gDNA）提取前，所有样本均保存于-80℃超低温环境中。本研究从米歇尔半岛（66°31'S, 110°59'E）采集3份土壤样本，从布朗宁半岛（66°27'S, 110°32'E）采集3份土壤样本。两处采样点的样本均沿样带布设，具体详细信息如下文所述。
宏基因组测序与组装：采用Nextera DNA Flex文库制备试剂盒（Nextera DNA Flex Library Prep Kit，Illumina公司）从群体基因组DNA提取物构建宏基因组鸟枪法文库。测序在Illumina NextSeq500平台上完成，使用2×150bp双端高通量输出测序化学试剂，每份样本的测序覆盖度为5GB。
ACE样本ID（含文件名内的ID）及对应土壤样本说明：
* SB9748- 土壤条形码= 91062- 原始条形码= 91604- 采样点= 布朗宁半岛- 样带编号= 3- 样本间距= 2m- 送检DNA浓度= 1.2ng/µL（达标浓度）- 送检DNA体积= 80µL
* SB9749- 土壤条形码= 91604- 原始条形码= 26587- 采样点= 布朗宁半岛- 样带编号= 3- 样本间距= 102m- 送检DNA浓度= 1ng/µL（达标浓度）- 送检DNA体积= 80µL
* SB9750- 土壤条形码= 91606- 原始条形码= 26617- 采样点= 布朗宁半岛- 样带编号= 3- 样本间距= 202m- 送检DNA浓度= 0.3ng/µL（达标浓度）- 送检DNA体积= 180µL
* SB9751- 土壤条形码= 91447- 原始条形码= 36260- 采样点= 米歇尔半岛- 样带编号= 2- 样本间距= 2m- 送检DNA浓度= 0.86ng/µL（达标浓度）- 送检DNA体积= 80µL
* SB9752- 土壤条形码= 91477- 原始条形码= 36290- 采样点= 米歇尔半岛- 样带编号= 2- 样本间距= 102m- 送检DNA浓度= 0.9ng/µL（达标浓度）- 送检DNA体积= 80µL
* SB9753- 土壤条形码= 91507- 原始条形码= 36320- 采样点= 米歇尔半岛- 样带编号= 2- 样本间距= 202m- 送检DNA浓度= 1.9ng/µL（达标浓度）- 送检DNA体积= 80µL