Uterine Influences on Conceptus Development in Fertility-Classified Animals

This study relates to pregnancy outcome after assisted reproduction of fertility-classified cattle. The aim is to investigate how the uterine environment impacts and programs conceptus survival and development. The study found that ripple effects of dysregulated conceptus-endometrial interactions elicit post-elongation pregnancy loss in subfertile animals during the implantation period. Overall design: Heifer cows classified as high fertile (HF), subfertile (SF), or infertile (IF) were investigated. The RNA-seq analysis was performed for endometrium samples at day 17 of pregnancy. For comparison, non-pregnant cows were included in the analysis. RNA from conceptus of HF and SF pregnant animals (day 17) were also included in the RNA-seq analysis. A total of 25 endometrium samples (5 non-pregnant of each fertilty group, 5 pregnant HF, and 5 pregnant SF) and 27 conceptus samples (10 SF and 17 HF) were used in the RNA-seq analysis.本研究聚焦于经生育力分级的牛（fertility-classified cattle）辅助生殖后的妊娠结局，旨在探究子宫微环境如何影响并调控胚胎（conceptus）的存活与发育。研究发现，胚胎-子宫内膜（conceptus-endometrial）互作失调所引发的级联效应，会诱发低生育力动物在着床期出现胚胎伸长后的妊娠丢失。 实验设计：本研究对按高生育力（high fertile，缩写HF）、低生育力（subfertile，缩写SF）及不育（infertile，缩写IF）分级的青年母牛开展研究。针对妊娠第17天的子宫内膜（endometrium）样本进行RNA测序（RNA-seq）分析；为设置对照，研究纳入了未妊娠母牛样本。此外，本研究还纳入了妊娠第17天高生育力与低生育力母牛的胚胎RNA样本，用于RNA测序分析。本次RNA测序分析共使用25份子宫内膜样本：每个生育力分级组的未妊娠样本5份、妊娠高生育力组样本5份、妊娠低生育力组样本5份；此外还使用了27份胚胎样本：低生育力组10份、高生育力组17份。