Nucleosome occupancy dynamics in yeast BY4741 responding to 0.4mM H2O2 over time (0-60 min)

Numerous factors have been implicated in regulating gene expression changes, including changes to nucleosome occupancy. Here we followed dynamic changes to nucleosome occupancy, gene expression and DNA binding of the transcription factor Msn2p genome-wide in yeast cells responding to hydrogen peroxide to reveal new relationships between regulators of stress-dependent gene expression. Nucleosome occupancy was measured by MNase-Seq in response to .4mM H2O2 in the S288c yeast derivative BY4741. A single replicate was performed for the time-course experiment with time points at 0, 4, 12, 20, 40 and 60 minutes after treatment.

诸多因素已被证实参与调控基因表达变化，其中包括核小体占据（nucleosome occupancy）状态的改变。本研究通过全基因组层面追踪酵母细胞在响应过氧化氢时的核小体占据状态、基因表达水平以及转录因子Msn2p的DNA结合动态变化，以揭示应激依赖型基因表达调控因子之间的新型关联。本研究在S288c酵母衍生株BY4741中，采用MNase-Seq技术检测0.4mM过氧化氢处理下的核小体占据状态。本次时间进程实验仅设置单次生物学重复，取样时间点分别为处理后0、4、12、20、40及60分钟。