Relative expression of pif1 (cDNA copies/µg RNA) in larvae infected with SfNIC-B and SfNIC-Bpif1 rescue viruses and SfNIC-Begt and SfNIC-Bp10 recombinant viruses.

Transcript amplifications were performed using qSfBpif.F and qSfBpif1.R primers. Target gene copy numbers were calculated based on SfMNPV genome molecular mass and the standard curve. Values indicate means ± SD of three different repetitions measured twice for each sample.qRT-PCR analysis of pif1 was performed on total RNA extracted from larvae infected with the different viruses at indicated times post-infection.采用qSfBpif.F与qSfBpif1.R引物进行转录扩增反应。靶基因拷贝数以SfMNPV基因组分子质量及标准曲线为基准进行计算。所得数值为每组样品设置3次独立重复、每次重复测定2次的平均值±标准差（standard deviation, SD）。以不同病毒感染的幼虫在感染后指定时间点提取的总RNA为模板，开展pif1基因的实时荧光定量逆转录PCR（quantitative reverse transcription PCR, qRT-PCR）分析。