Supplemental_Data_JFWM-108R

Table A1. The results of a fall Chinook salmon (Oncorhynchus tshawytscha) passage and handling study conducted in November, 2014, at the Priest Rapids Salmon Hatchery, Mattawa, Washington. Each adult salmon was given a Passive Integrated Transponder (PIT) tag with a unique identification code (column: PIT tag number). Their gender was determined (column: Sex with M = male and F = female). Fork length was measured in cm (column: Length), body circumference was measured in cm (column: Circumference), and body weight taken in kg (column: Weight). Fish were divided into one of four treatment groups (column: Treatment) – Whoosh Fish Transport System (WFTS) 77 m tube (WFTS-77), WFTS 12 m tube (WFTS-12), trap and haul (T&H), or control. Treatment dates (column: Treatment date) ranged from November 4-6, 2014 and mortality dates (column: Mortality date) ranged from November 4-11, 2014. The following information was collected on a subset of fish – column: Spleen IL1-β normalized with 18s (n = 65); column: Spleen IgM normalized with 18s (n = 65); column: Cortisol concentration (ng/mL) (n = 41); column: Day 3 vitellogenin concentration (mg/mL) (n = 63); and column: Percent epithelial injury (%) (n = 42). In all columns, NR represents no data were recorded.
Table A2. The gamete survival results from a study on the effects of passage and handling conducted in 2014 with adult fall chinook salmon (Oncorhynchus tshawytscha) that were captured at Priest Rapids Salmon Hatchery, Mattawa, Washington. Each fish was given a Passive Integrated Transponder (PIT) tag with a unique identification code (column: PIT tag number). Fish were divided into one of four treatment groups (column: Treatment) – Whoosh Fish Transport System (WFTS) 77 m tube (WFTS-77), WFTS 12 m tube (WFTS-12), trap and haul (T&H), or control. Their gender was determined (column: Sex with M = male and F = female). One female’s eggs were fertilized with sperm from one male (column: Pair number) for a total of 37 pairings (i.e., sib family). The fertilized eggs were divided into three sub-samples per family (approximately 100 eggs per sub-sample) and randomly assigned to a stack, tray and cell in vertical flow incubators. Fertilized eggs were held in incubation trays at ~10°C until they reached the eyed egg stage (December 17, 2014, or approximately 40 days post-fertilization), at which time the number of live and dead eggs were counted to determine gamete survival by cell (columns labeled 1st cell survival, 2nd cell survival, and 3rd cell survival, all percentages). Data analysis was done on the average embryo survival to the eyed stage (subsamples were averaged per sib family) (column: Average survival %).

表A1：2014年11月于华盛顿州马塔瓦市普里斯特拉皮兹鲑鱼孵化场开展的秋季奇努克鲑（Oncorhynchus tshawytscha）过坝与处理实验结果。
每尾成年试验鱼均被植入带有唯一识别码的被动集成应答器（Passive Integrated Transponder，PIT）标签，对应数据列记为「PIT标签编号」。通过观测确定其性别，对应数据列记为「性别」，其中M代表雄性，F代表雌性。测量其叉长（单位：厘米，对应数据列记为「叉长」）、体围（单位：厘米，对应数据列记为「体围」）与体质量（单位：千克，对应数据列记为「体质量」）。试验鱼被分为4个处理组之一，对应数据列记为「处理组」，分别为：快速鱼类运输系统（Whoosh Fish Transport System，WFTS）77米管道组（WFTS-77）、WFTS 12米管道组（WFTS-12）、诱捕转运组（Trap and Haul，简称T&H）以及对照组。处理日期对应数据列记为「处理日期」，范围为2014年11月4日至6日；死亡日期对应数据列记为「死亡日期」，范围为2014年11月4日至11日。
针对部分试验鱼采集了以下指标数据：以18S rRNA为内参的脾脏IL-1β表达量（n=65，对应数据列记为「脾脏IL-1β（18S归一化）」）、以18S rRNA为内参的脾脏IgM表达量（n=65，对应数据列记为「脾脏IgM（18S归一化）」）、皮质醇浓度（单位：纳克/毫升，n=41，对应数据列记为「皮质醇浓度」）、第3天卵黄蛋白原浓度（单位：毫克/毫升，n=63，对应数据列记为「第3天卵黄蛋白原浓度」）以及上皮损伤百分比（单位：%，n=42，对应数据列记为「上皮损伤率」）。所有数据列中，「NR」代表未记录相关数据。

表A2：2014年针对在华盛顿州马塔瓦市普里斯特拉皮兹鲑鱼孵化场捕获的成年秋季奇努克鲑（Oncorhynchus tshawytscha）开展的过坝与处理实验，其配子存活率结果。每尾试验鱼均被植入带有唯一识别码的PIT标签，对应数据列记为「PIT标签编号」。试验鱼被分为4个处理组之一，对应数据列记为「处理组」，分别为WFTS 77米管道组（WFTS-77）、WFTS 12米管道组（WFTS-12）、诱捕转运组（T&H）以及对照组。性别鉴定结果记于「性别」列，其中M代表雄性，F代表雌性。采用1尾雄鱼的精液对1尾雌鱼的卵进行受精，形成37组配对组合（即全同胞家系），对应数据列记为「配对编号」。每个家系的受精卵被分为3个亚样本（每个亚样本约100枚卵），并被随机分配至垂直流孵化器的组、盘与单元中。受精卵在约10℃的孵化盘中培育，直至达到发眼卵阶段（2014年12月17日，即受精后约40天），此时统计活卵与死卵数量，以计算每个孵化器单元的配子存活率，对应数据列记为「第1单元存活率」「第2单元存活率」「第3单元存活率」，均以百分比表示。以每个全同胞家系的亚样本存活率平均值为基础，计算胚胎至发眼卵阶段的平均存活率，对应数据列记为「平均存活率（%）」。