Whole Transcriptome analysis for porcine alveolar macrophage infected by African Swine Fever Virus

African swine fever virus (ASFV) is one of the most devastating swine pathogens characterized by nearly 100% mortality in naive herds and was recently emerged the in China. In this study, we generated the expression profile of porcine alveolar macrophages (PAMs) infected with a high pathogenic ASFV (Pig/Heilongjiang/2018 (Pig/HLJ/18) ASFV). Our data indicated that ASFV infection lead to a strong inhibition of host immunity but promote chemokine-mediated signaling pathway and neutrophil chemotaxis. Moreover, ASFV infection can modulate the host miRNA involved regulation network, leading to a significant increase of host metabolism related genes and acceleration of virus replication. Furthermore, ASFV-derived viral small RNAs (vsRNAs) can target some host immune response related genes. In conclusion, our transcriptome-wide data provide some insights into the regulatory mechanism during ASFV infection. MicroRNA and mRNA profiles of porcine alveolar macrophages (PAMs) at different time-points post ASFV infection. MicroRNA and mRNA datasets were generated by deep sequencing, in triplicate, using Illumina Nextseq CN500 and Hiseq Xten platforms, respectively.

非洲猪瘟病毒（African swine fever virus, ASFV）是极具破坏性的猪类病原体之一，其在易感猪群中的致死率近乎100%，近期在中国出现暴发。本研究针对高致病性非洲猪瘟病毒（Pig/Heilongjiang/2018，简称Pig/HLJ/18 ASFV）感染的猪肺泡巨噬细胞（porcine alveolar macrophages, PAMs）构建了表达谱。研究数据显示，非洲猪瘟病毒感染可强烈抑制宿主免疫应答，同时激活趋化因子介导的信号通路与中性粒细胞趋化过程。此外，非洲猪瘟病毒感染可调控宿主microRNA（miRNA）参与的调控网络，使得宿主代谢相关基因表达显著上调，并加速病毒复制。进一步研究发现，非洲猪瘟病毒来源的病毒小RNA（viral small RNAs, vsRNAs）可靶向调控部分宿主免疫应答相关基因。综上，本研究获得的全转录组数据为解析非洲猪瘟病毒感染过程中的调控机制提供了新视角。本数据集包含非洲猪瘟病毒感染后不同时间点的猪肺泡巨噬细胞microRNA与信使RNA（mRNA）表达谱。microRNA与mRNA数据集分别通过Illumina Nextseq CN500与Illumina Hiseq Xten平台进行三次重复深度测序构建。