CIL:1438

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:1437.

本数据集的样品为兔腰大肌骨骼肌纤维，先将其维持于恒定长度，再通过浸入甘油诱发肌僵直。随后在Heuser型冷冻压榨仪（cryopress）中，使纤维与液氦冷却铜块接触以实现快速冷冻；接着将样品置于Balzers 400型冷冻断裂仪（freeze fracture machine）中进行冷冻蚀刻处理。之后以铂碳（Pt-C）在24°倾角下对样品进行旋转复型，再在加速电压为100千电子伏的JEOL 100CX2型透射电子显微镜（transmission electron microscope）下完成成像观察。图像以68000倍的放大倍率记录于柯达4489胶片上，并拍摄了±5°倾角的立体图像对。随后使用尼康Coolscan 9000ED扫描仪，以20微米的像素间距对胶片进行扫描。该立体图像对的编号为CIL:1437。