Metabolic characteristics of different flue-cured tobacco varieties and grades and quality improvement effects of nicotine-degrading bacteria on tobacco leaves

To investigate the metabolite differences among different varieties and grades of flue-cured tobacco, as well as the impacts of nicotine-degrading bacteria on the quality of different grades of tobacco leaves. This study employed untargeted metabolomics to identify and analyze metabolites in fermented tobacco leaves of ‘Yunyan 87’ and ‘Yunyan 97’, specifically B2F and C2F grades, while also examining the influences of nicotine-degrading bacteria on the quality of different grades of flue-cured tobacco leaves. [Results] There were significant metabolite differences between tobacco leaves of different varieties and grades. A total of 131 differential metabolites were identified between the samples of ‘Yunyan 87’ and ‘Yunyan 97’, while 138 differential metabolites were identified between B2F and C2F grades.

为探究不同品种、等级烤烟（flue-cured tobacco）的代谢物差异，以及尼古丁降解菌（nicotine-degrading bacteria）对不同等级烟叶品质的影响。本研究采用非靶向代谢组学（untargeted metabolomics）技术，对‘云烟87’与‘云烟97’两个烤烟品种的B2F、C2F等级发酵烟叶中的代谢物进行鉴定与分析，同时考察尼古丁降解菌对不同等级烤烟叶片品质的影响。【结果】不同品种与等级的烟叶间存在显著代谢物差异。‘云烟87’与‘云烟97’的样本间共鉴定出131种差异代谢物，B2F与C2F等级的样本间则鉴定出138种差异代谢物。