Transcriptomic analysis of SRSF1 overexpression and imatinib treatment in K562 CML cell lines

Using RNA-sequencing, we report the effects of overexpressing the proto-oncogene SRSF1 in K562 cells on gene expression and alternative splicing. We found that overexpressing SRSF1 antagonized the gene expression changes brought about by imatinib treatment. Overall design: K562 cells stably expressing either the empty vector or SRSF1 gene. Treated with 0.5uM imatinib for 48 h.

本研究通过RNA测序（RNA-sequencing）技术，报道了在K562细胞中过表达原癌基因（proto-oncogene）SRSF1对基因表达与可变剪接（alternative splicing）的影响。研究发现，SRSF1过表达会拮抗伊马替尼（imatinib）处理所引发的基因表达变化。实验整体设计如下：将分别稳定表达空载体或SRSF1基因的K562细胞，以0.5μM伊马替尼处理48小时。