Correlation of the kinetics of viral antigen and genomic RNA with restoration of normal cell homeostasis

The main objective of the studies within COCID Work Package 6 is to understand basic mechanisms by which viral replication machineries are removed from cells after pharmacological interruption of viral replication using functional as well as imaging techniques, including soft X-ray tomography.

In this document, we highlight the progress in establishing the HCV replication models (replicons), the antiviral treatment chosen to eliminate viral structures from the host cells and the correlation between elimination of the viral replication machinery and restoration of a normal host cell homeostasis, using markers of HCV-induced stress. These data are essential to frame the experimental setup chosen for the imaging process required in subsequent stps of the project.

COCID工作包6内各项研究的核心目标，是解析通过药理学手段阻断病毒复制后，借助功能技术与成像技术（包括软X射线断层扫描（soft X-ray tomography））清除细胞内病毒复制机器的基本机制。

本文件重点阐述了以下三方面的研究进展：丙型肝炎病毒（Hepatitis C Virus, HCV）复制模型（复制子，replicons）的构建、用于清除宿主细胞内病毒结构的抗病毒治疗方案，以及利用HCV诱导的应激标志物分析病毒复制机器清除与宿主细胞正常稳态恢复之间的关联。上述数据可为该项目后续阶段所需成像流程的实验框架设计提供关键依据。