3D models of the TBSV replication platform as visualized by Metal-Tagging Transmission Electron Microscopy (METTEM) and Electron Tomography (ET)

Movies S1-S2. 3D models of the TBSV replication platform as visualized by METTEM and ET (from tomograms in Fig. 5A and Fig. 6A). Movie S3. 3D model of a TBSV replication platform as visualized by METTEM immunogold labeling with anti-dsRNA antibodies and ET (from volume in Fig. 5B). The computational slices of the tomogram are first swept upwards (first third of the movie) then backwards (second third), revealing the 3D isosurface representation. The last third of the movie rotates the 3D representation. Movie S4. 3D model of a TBSV replication platform as visualized by METTEM immunogold labeling with anti-dsRNA antibodies and ET (from volume in Fig. 7). The computational slices of the tomogram are first swept upwards (first third of the movie) then backwards (second third), revealing the 3D isosurface representation. The last third of the movie rotates the 3D representation.影片集 S1-S2：基于图5A与图6A中的断层扫描图，通过METTEM与电子断层扫描（Electron Tomography, ET）技术可视化得到的TBSV复制平台3D模型。 影片 S3：基于图5B中的断层扫描数据，通过使用抗双链RNA（double-stranded RNA, dsRNA）抗体进行免疫金标记的METTEM技术与ET技术可视化得到的TBSV复制平台3D模型。本影片首先向上扫动断层扫描图的计算切片（影片前1/3部分），随后向后扫动（影片中1/3部分），以展示3D等值面（isosurface）可视化结果。影片最后1/3部分对该3D模型进行旋转展示。 影片 S4：基于图7中的断层扫描数据，通过使用抗双链RNA（double-stranded RNA, dsRNA）抗体进行免疫金标记的METTEM技术与ET技术可视化得到的TBSV复制平台3D模型。本影片首先向上扫动断层扫描图的计算切片（影片前1/3部分），随后向后扫动（影片中1/3部分），以展示3D等值面（isosurface）可视化结果。影片最后1/3部分对该3D模型进行旋转展示。