Can intramammary infections change immunoglobulins and acute phase proteins of colostrum and transition milk in dairy goats?

ABSTRACT Proteiongram analysis is useful for the early diagnosis of intramammary infections during the period of colostrogenesis. This study aimed to evaluate the profile of total proteins, immunoglobulins, lactoferrin, and gamma-glutamyl transferase (GGT) in the colostrum of dairy goats with intramammary infections. Animals were divided in groups: GI (n=12) of goats without mammary gland infections, and GII (n=8) of goats with mammary gland infections. Intramammary infections were diagnosed using microbiological isolations and somatic cell counts (SCCs). Total protein was evaluated in the samples using SDS-PAGE shortly after parturition, and 24 and 48 hours after that event. Non-aureus Staphylococcus (NAS) were detected in all isolates. At 48 h, GII had high IgG levels and a SCC of 1660.25 × 10³/mL. Levels of total protein were high in this group at 24 and 48 h. Albumin levels were high in goats with mastitis at 24 h. Overall, the IgG, lactoferrin, and albumin levels differed between animals with and without intramammary infections at M0. GGT activity was not influenced by the intramammary infection. The results of this study reinforce the importance of the proteinogram as an auxiliary tool in the diagnosis of mastitis in dairy goats.

摘要 初乳生成期的蛋白图谱分析（Proteiongram analysis）可用于乳腺内感染的早期诊断。本研究旨在评估患有乳腺内感染的奶山羊初乳中总蛋白、免疫球蛋白、乳铁蛋白及γ-谷氨酰转移酶（gamma-glutamyl transferase, GGT）的表达谱。将试验动物分为两组：GI组（n=12）为无乳腺感染的奶山羊，GII组（n=8）为患有乳腺感染的奶山羊。乳腺内感染通过微生物分离培养与体细胞计数（somatic cell counts, SCCs）进行确诊。分别于分娩后即刻、分娩后24 h及48 h采集样本，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）检测总蛋白水平。所有分离菌株均为非金黄色葡萄球菌（Non-aureus Staphylococcus, NAS）。分娩后48 h时，GII组IgG水平升高，体细胞计数达1660.25×10³/mL。该组在分娩后24 h及48 h的总蛋白水平均较高。分娩后24 h时，患乳腺炎的奶山羊白蛋白水平升高。总体而言，在M0时间点，患有与未患有乳腺内感染的奶山羊的IgG、乳铁蛋白及白蛋白水平存在显著差异。γ-谷氨酰转移酶活性不受乳腺内感染的影响。本研究结果进一步证实，蛋白图谱分析作为奶山羊乳腺炎辅助诊断工具的重要价值。