HiC/TCC data of barley (H. vulgare) plants. HiC/TCC

We employed chromosome conformation capture sequencing (HiC) and its derivative protocol tethered chromosome conformation capture (TCC) to interrogate the three-dimensional conformation of barley chromatin in the nucleus. These data were used (i) for chromosome-scale sequence scaffolding in frame of the International Barley Genome Sequencing Consortium's effort to construct a map-based reference sequence assembly of barley cv. "Morex" and (ii) to describe the three-dimensional conformation of the barley genome at megabase resolution on the basis of this genome sequence.

本研究采用染色体构象捕获测序（HiC，chromosome conformation capture sequencing）及其衍生方案锚定染色体构象捕获（TCC，tethered chromosome conformation capture），对细胞核内大麦染色质的三维构象进行解析。所获数据可用于两项研究：（i）在国际大麦基因组测序联盟（International Barley Genome Sequencing Consortium）构建大麦栽培品种"Morex"的基于图谱的参考基因组组装工作的框架下，完成染色体尺度的序列挂载；（ii）基于该基因组序列，以兆碱基分辨率描述大麦基因组的三维构象。