circadian cycle-The impact of some genes mutations implicated in the amidon metabolism. Arabidopsis thaliana

af30_starch_circadian_rythm - circadian cycle - The scientific aim is to study the impact of some genes mutations implicated in the amidon metabolism on the expression profils of other genes and in particular those of the carbonated metabolism. Those analyses will be done from RNAs (total or messenger) extracted from leaves at various time of the day and the night - The first experiments will deal with the wild reference plant (WS) . The RNAm of leaves would be harvested according to day and night hours. Some harvesting will be done every 3 hours during a 24H period (so 10 different points ). This correspond to a total of 20 slides ( 2 samples and 1 swap per point). This account have been done according to the informations obtained from JJ Leguay in dec 2002 Keywords: time course Overall design: 10 dye-swap - CATMA arrays

af30_starch_circadian_rythm - 昼夜节律周期（circadian cycle）：本数据集的科学目标为探究参与淀粉代谢的部分基因突变对其他基因，尤其是碳代谢相关基因的表达谱的影响。相关分析将以提取自昼夜不同时段叶片的RNA（总RNA或信使RNA（messenger RNA））为材料开展。首批实验将以野生型参考植株（WS）为对象，依据昼夜时段采集叶片的mRNA：部分采样在24小时周期内每3小时进行1次，共计10个采样时间点。每个时间点对应2个样本及1次染料交换，总计20张芯片。本实验设计基于2002年12月JJ Leguay提供的相关信息。关键词：时间进程（time course）。整体实验设计：10次染料交换——CATMA阵列（CATMA arrays）