16S rRNA gene sequencing on swine hindgut microbiota after xylanase supplementation

This research tested the hypothesis that xylanase modulates microbial communities within the large intestine of growing pigs fed insoluble corn-based fiber through a stimbiotic mechanism of action. Sixty gilts were blocked by initial body weight, individually-housed, and randomly assigned to 1 of 4 dietary treatments (n=15): a low-fiber control (LF), a high-fiber control containing 30% corn bran without solubles (HF), HF+100 mg/kg xylanase (HF+XY), and HF+50 mg/kg arabinoxylan oligosaccharide (HF+AX). Pigs were fed their respective dietary treatments for 46-days. On day 46, pigs were euthanized, and mucosa and contents were collected from the apex of the cecum and the proximal colon. The V4 region of bacterial 16S rRNA gene was amplified and sequenced.

本研究验证了如下假说：木聚糖酶（xylanase）可通过促生素（stimbiotic）作用机制，对饲喂不溶性玉米基纤维的生长猪大肠内的微生物群落进行调控。本试验选取60头后备母猪，以初始体重为依据进行区组分组，单栏饲养后随机分配至4个日粮处理组（每组n=15）：低纤维对照组（LF）、含30%去可溶性成分玉米麸皮的高纤维对照组（HF）、HF+100 mg/kg木聚糖酶组（HF+XY）以及HF+50 mg/kg阿拉伯木聚糖寡糖（arabinoxylan oligosaccharide）组（HF+AX）。试验猪按对应日粮处理饲喂46天。第46天时，对试验猪实施安乐死，采集盲肠顶端与近端结肠的黏膜及肠道内容物样本。对细菌16S rRNA基因的V4区域进行扩增并测序。